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The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I

Paper ID Volume ID Publish Year Pages File Format Full-Text
17777 42695 2008 5 PDF Available
Title
The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I
Abstract

Protein adsorption onto solid substrates usually takes place in an irreversible fashion and this irreversible adsorption also occurs in some enzymatic reactions. In this work the adsorption behavior of intact β-1, 4-glucan-cellobiohydrolase (CBH I) from Trichoderma reesei onto microcrystalline cellulose was monitored by surface plasmon resonance and UV-spectral method. It was found that there existed reversible binding and irreversible binding of CBH I during its interaction with cellulose substrate. To evaluate the influence of adsorption on cellulose enzymatic hydrolysis, the reaction dynamics on pure cellulose were determined. A plot of the hydrolysis rate against the surface density of irreversibly adsorbed CBH I, revealed an inverse relationship in which an apparent decrease in the hydrolysis rate was observed with increasing surface density. Taken together, results presented here should be useful for modifying the binding characteristics of CBH I and making them more effective in cellulose hydrolysis.

Keywords
Cellobiohydrolase I; Hydrolysis; Irreversible adsorption; Solid–liquid interface; Rate retardation
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The enzymatic hydrolysis rate of cellulose decreases with irreversible adsorption of cellobiohydrolase I
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 7, 5 June 2008, Pages 543–547
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us