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Metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule

Paper ID Volume ID Publish Year Pages File Format Full-Text
17782 42695 2008 7 PDF Available
Title
Metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule
Abstract

Microencapsulation technology is a convenient method to alter and regulate cell product formation. In order to probe the metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule, the hyper-osmo-sensitive type S. cerevisiae (Y02724) and wild type S. cerevisiae (BY4741) were encapsulated into liquid core ACA microcapsules. The behavior of cell growth, glucose consumption, ethanol production and the yields of glycerol and organic acids were determined. Free cell culture was used as control. The enzyme activities of NADP+-glutamate dehydrogenase (GDH), glutamine synthetase (GS) and glutamate synthase (GOGAT) on microencapsulation cells and free cultured cells were measured too. The results demonstrated that the growth of Y02724 in both aerobic and anaerobic conditions was seriously inhibited by ACA microcapsule, while the ethanol and acetatic acid yield of microencapsulation Y02724 in anaerobic condition were significantly higher than that of suspended cultivation. For Y02724, the microencapsulation cultivation significantly increased the GS and GOGAT activities and decreased the GDH activity in comparison with control group. ACA microcapsules did not significantly change the growth behavior and metabolic performance of BY4741, but decreased the GS activity. In conclusion, microcapsules microenvironment significantly changes the metabolism behavior of hyper-osmo-sensitive type S. cerevisiae (Y02724), but nearly had no effect on BY4741.

Keywords
Sacchromyces cerevisiae; Osmo-sensitive; ACA microcapsules; Metabolism
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Metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 7, 5 June 2008, Pages 576–582
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us