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Anhydrous tert-pentanol as a novel media for the efficient enzymatic synthesis of amoxicillin

Paper ID Volume ID Publish Year Pages File Format Full-Text
17786 42695 2008 7 PDF Available
Title
Anhydrous tert-pentanol as a novel media for the efficient enzymatic synthesis of amoxicillin
Abstract

The efficient enzymatic synthesis of amoxicillin using anhydrous tert-pentanol as a novel media has been demonstrated for the first time. p-OH-Phenylglycine methyl ester (HPGM) was selected as the activated acyl donor due to its good solubility in organic solvents. The screening results of 21 organic solvents showed that solvents with either strong polarity or poor substrate solubility were unfavorable. Remarkable catalytic activity of the immobilized penicillin acylase (IPA) from Escherichia coli was retained in tert-pentanol, and high yield could be obtained. Effects of various parameters such as acyl donor, water content or cosolvents of tert-pentanol, substrate concentration, temperature, etc., on the enzymatic synthesis of amoxicillin in tert-pentanol were investigated systematically. The best reaction medium, the optimal temperature, initial concentration of 6-APA and HPGM and concentration of enzyme were tert-pentanol, 15 °C, 100, 200 mM and 20 IU/mL, respectively. Under the optimal conditions, the yield of amoxicillin was as high as 88% after a reaction time of 20 h.

Keywords
Amoxicillin; Enzymatic synthesis; Organic solvent; Penicillin G acylase
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Anhydrous tert-pentanol as a novel media for the efficient enzymatic synthesis of amoxicillin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 7, 5 June 2008, Pages 601–607
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us