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Synthesis and characterization of hydroquinone glucoside using Leuconostoc mesenteroides dextransucrase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17804 42697 2009 6 PDF Available
Title
Synthesis and characterization of hydroquinone glucoside using Leuconostoc mesenteroides dextransucrase
Abstract

We synthesized a hydroquinone glucoside (HG) as a potential skin-whitening agent using Leuconostoc mesenteroides (B-1299CB BF563) dextransucrase with hydroquinone (HQ) as an acceptor and sucrose as a donor. The product was purified using butanol partitioning and silica gel column chromatography. The structure of the purified HG was determined by nuclear magnetic resonance and the ionic product was observed at m/z 295 (C12, H16, O7 Na)+. HG was identified as 4-hydroxyphenyl-α-d-glucopyranoside. The optimum condition of HG synthesis, determined using a response surface methodology, was 450 mM HQ, 215 mM sucrose, and 0.55 U/mL dextransucrase; the final HG produced was 544 mg/L. The IC50 of diphenylpicryl-hydrazyl scavenging activity was 3.85 mM indicating a higher antioxidant activity compared to β-arbutin (IC50 = 6.04 mM). HG-mediated inhibition of lipid peroxidation was 3.51% that of HQ (100%) and much higher than that of β-arbutin (0.81% of HQ). In addition the IC50 value of nitrite-scavenging activity was 14.76 mM showing a superior scavenging activity to that of β-arbutin (IC50 = 27.09 mM).

Keywords
Leuconostoc mesenteroides; Dextransucrase; Hydroquinone; Acceptor reaction; Glucosylation; Antioxidant
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Synthesis and characterization of hydroquinone glucoside using Leuconostoc mesenteroides dextransucrase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 5, 5 November 2009, Pages 355–360
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us