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Effects of galactomannan as carbon source on production of α-galactosidase by Thermomyces lanuginosus: Fermentation, purification and partial characterisation

Paper ID Volume ID Publish Year Pages File Format Full-Text
17806 42697 2009 5 PDF Available
Title
Effects of galactomannan as carbon source on production of α-galactosidase by Thermomyces lanuginosus: Fermentation, purification and partial characterisation
Abstract

Thermophilic fungus Thermomyces lanuginosus CBS 395.62/b strain is able to grow and synthesise extracellular α-galactosidase in media containing galactomannan such as locust bean gum (LBG) or guar gum (GG). Production of extracellular α-galactosidase was enhanced from 1.2 U/mL to 4–6 U/mL meaning about 3–5 times increase by optimisation of medium composition. This enzyme was purified to homogeneity by partial precipitation with 2-propanol and different liquid chromatographical steps. The developed purification protocol yielded 22% of enzyme activity with 900 purified fold. Molecular mass of the purified α-galactosidase enzyme was estimated to be 53 kDa. Maximal catalytic activity of the enzyme was obtained in the acidic pH range between pH 4.6 and 4.8 and in the temperature range 60–66 °C. More than 95% of enzyme activity was remaining after 1-day incubation at 70 °C and on pH in the range from 4.0 to 7.0. The enzyme activity was significantly stimulated by Mg2+, Mn2+ and K+ ions, while considerably inhibited by the presence of Ca2+, Ag+ and Hg2+.

Keywords
α-Galactosidase; Galactomannan; Thermomyces lanuginosus; Thermophilic fungus
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Effects of galactomannan as carbon source on production of α-galactosidase by Thermomyces lanuginosus: Fermentation, purification and partial characterisation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 5, 5 November 2009, Pages 367–371
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us