fulltext.study @t Gmail

Coupled oxidation–reduction of butanol–hexanal by resting Rhodococcus erythropolis NCIMB 13064 cells in liquid and gas phases

Paper ID Volume ID Publish Year Pages File Format Full-Text
17818 42698 2008 8 PDF Available
Title
Coupled oxidation–reduction of butanol–hexanal by resting Rhodococcus erythropolis NCIMB 13064 cells in liquid and gas phases
Abstract

Rhodococcus erythropolis is a promising Gram-positive bacterium capable of numerous bioconversions including those involving alcohol dehydrogenases (ADHs). In this work, we compared and optimized the redox biocatalytic performances of 1-butanol-grown R. erythropolis NCIMB 13064 cells in aqueous and in non-conventional gas phase using the 1-butanol–hexanal oxidation–reduction as model reaction. Oxidation of 1-butanol to butanal is tightly coupled to the reduction of hexanal to 1-hexanol at the level of a nicotinoprotein–ADH-like enzyme. Cell viability is dispensable for reaction. In aqueous batch conditions, fresh and lyophilized cells are efficient redox catalysts (oxidation–reduction rate = 765 μmol min−1 g cell dry mass−1) being also reactive towards benzyl alcohol, (S)-2-pentanol, and geraniol as reductants. However, butanol–hexanal oxidation–reduction is strongly limited by product accumulation and by hexanal toxicity that is a major factor influencing cell behavior and performance. Reaction rate is maximal at 40 °C-pH 7.0 in aqueous phase and at 60 °C-pH 7.0–9.0 in gas phase. Importantly, lyophilized cells also showed to be promising redox catalysts in the gas phase (at least 65 μmol min−1 g cell dry mass−1). The system is notably stable for several days at moderate thermodynamic activities of hexanal (0.06–0.12), 1-butanol (0.12) and water (0.7).

Keywords
Biocatalysis; Whole cells; Oxido-reduction; Rhodococcus erythropolis; Liquid phase; Solid/gas phase
First Page Preview
Coupled oxidation–reduction of butanol–hexanal by resting Rhodococcus erythropolis NCIMB 13064 cells in liquid and gas phases
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 6, 6 November 2008, Pages 423–430
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us