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Growth and metabolism of a meat isolated strain of Pediococcus pentosaceus in submerged fermentation: Purification, characterization and properties of the produced pediocin SM-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
17822 42698 2008 7 PDF Available
Title
Growth and metabolism of a meat isolated strain of Pediococcus pentosaceus in submerged fermentation: Purification, characterization and properties of the produced pediocin SM-1
Abstract

The meat isolate Pediococcus pentosaceus, characterized as P. pentosaceus Mees 1934, produces a bacteriocin which was characterized in this study. The molecule was found to have the pediocin characteristics (with respect to N-terminal sequence and the presence of the characteristic consensus motif –YGNGV–), a molecular mass of 5.370 Da, and was designated as pediocin SM-1. The new bacteriocin is inhibitory to several food spoilage and food-born pathogens, shows a remarkable stability to heat and cold treatments, as well as to a wide pHs range, but it appears to be sensitive to proteases. Its mode of action appears to be bactericidal. Fermentation studies carried out in a stirred tank bioreactor revealed that semi-aerobic conditions (60% dissolved oxygen saturation) enhance production and fermentation rate analysis indicate primary metabolite kinetics for growth under the particular conditions. Pediocin SM-1, produced by a food-grade microorganism, exhibits the characteristics of a potential biopreservative and can be efficiently produced by fermentation.

Keywords
Pediococcus pentosaceus; Pediocin; Bacteriocin; Biopreservative
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Growth and metabolism of a meat isolated strain of Pediococcus pentosaceus in submerged fermentation: Purification, characterization and properties of the produced pediocin SM-1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 6, 6 November 2008, Pages 448–454
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us