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Production, purification and characterization of an alkaliphilic endo-β-1,4-xylanase from a microbial community EMSD5

Paper ID Volume ID Publish Year Pages File Format Full-Text
17829 42699 2008 6 PDF Available
Title
Production, purification and characterization of an alkaliphilic endo-β-1,4-xylanase from a microbial community EMSD5
Abstract

In this study, a novel alkaliphilic extracellular endo-β-1,4-xylanase was purified from the culture filtrate of microbial community EMSD5, which could grow in liquid culture fermentation under static condition with corn stover as inducement. Single step purification was achieved using anion exchange chromatography. The purified enzyme showed a single band on SDS polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular weight of 22 kDa and pI of 7.1. Stimulation of the activity by β-mercaptoethanol, and dithiotheritol (DTT) was observed. Moderately thermostable xylanase showed optimum activity at 50 °C in pH 8.0. 16S rDNA clone library analysis indicated this community was mainly composed of bacteria related to genera Clostridium, Acinetobacter, Bacteroides, Lysinibacillus and Dysgonomonas. The present work reports for the first time the purification and characterization of an alkaliphilic xylanase from a microbial community, and the alkaliphilicity and moderate thermostability of this xylanase may have potential implications in pulp and paper industries.

Keywords
Microbial community; Alkaliphilic xylanase; Corn stover; SDS-PAGE; Enzyme; Purification
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Production, purification and characterization of an alkaliphilic endo-β-1,4-xylanase from a microbial community EMSD5
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issues 4–5, 6 October 2008, Pages 343–348
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us