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Response surface methodology: Synthesis of inulooligosaccharides with an endoinulinase from Aspergillus niger

Paper ID Volume ID Publish Year Pages File Format Full-Text
17832 42699 2008 7 PDF Available
Title
Response surface methodology: Synthesis of inulooligosaccharides with an endoinulinase from Aspergillus niger
Abstract

Oligosaccharides of chain length up to degree of polymerisation (DP) 5 have been synthesised using a purified endoinulinase and response surface methodology with central composite experimental design for the optimisation of the product yield. The endoinulinase was purified through a three-step procedure in a yield of 1.11% and 3.5-fold and its molecular weight was 68.1 kDa by SDS-PAGE. It had optimum pH and temperature values of 6 and 60 °C, respectively with only 10% loss in activity over 60 min under these conditions. From the kinetic studies Km and Vmax were estimated at 3.53 mM and 666.7 μmol min−1 ml−1, respectively. Using the purified endoinulinase preparation isolated from Aspergillus niger, at a temperature (60 °C), inulin concentration (150 mg ml−1), incubation time (48 h), pH (6.0) and enzyme dosage (60 U ml−1) produced high yields of inulotrioses (70.3 mM), inulotetraoses (38.8 mM), and inulopentaoses (3.5 mM). Response surface regression predicted similar product levels under the same conditions. A comparative analysis of the yields of individual oligosaccharides produced from both the crude and purified enzyme is also presented.

Keywords
Response surface methodology; Endoinulinase; Synthesis; Inulooligosaccharides
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Response surface methodology: Synthesis of inulooligosaccharides with an endoinulinase from Aspergillus niger
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issues 4–5, 6 October 2008, Pages 362–368
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us