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Optimizing vitamin B12 biosynthesis by mycelial cultures of Lentinula edodes (Berk.) Pegl.

Paper ID Volume ID Publish Year Pages File Format Full-Text
17833 42699 2008 6 PDF Available
Title
Optimizing vitamin B12 biosynthesis by mycelial cultures of Lentinula edodes (Berk.) Pegl.
Abstract

Preparations derived from Lentinula edodes (Berk.) Pegl. are widely used as dietary supplements, they contain compounds with immune system enhancing and chemopreventive properties. These preparations contain a blend of minerals and vitamins, including vitamins A, D, B1, B2, C, and niacin, but only scant vitamin B12. Our goal was to optimize the growth conditions of submerged mycelial cultures of L. edodes in order to obtain a new dietary supplement enriched in vitamin B12. We designed a biotechnological process in which cobalamin precursors, cobalt chloride and the methyl donors betaine, methionine, and choline, were added to the culture medium. The vitamin B12 content in mycelial extracts was determined by RP-HPLC, while total Co2+ content was determined by HPIC. At the optimal Co2+ concentration (40 μg/mL), the vitamin B12 level in L. edodes mycelium reached 95 μg/g of dry weight. All methyl donors had a positive effect on cobalamin biosynthesis, at least doubling its concentration in mycelia as compared mycelia grown in non-enriched medium. The amount of cobalamin in submerged cultivated mycelia of L. edodes was 10,000-fold higher than that recorded for fruiting bodies. Our results suggest that these optimized culture conditions could be applied to obtain a new cobalamin-enriched dietary supplement derived from L. edodes.

Keywords
Betaine; Methionine; Choline; Lentinula edodes; Vitamin B12
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Optimizing vitamin B12 biosynthesis by mycelial cultures of Lentinula edodes (Berk.) Pegl.
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issues 4–5, 6 October 2008, Pages 369–374
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us