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Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica

Paper ID Volume ID Publish Year Pages File Format Full-Text
17839 42700 2008 7 PDF Available
Title
Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica
Abstract

The xylanase gene from the rumen fungus Neocallimastix frontalis was expressed in Pichia pastoris and Pichia methanolica. Using a complex medium with medium replacement before induction and the maintenance of the methanol induction level at 0.5%, P. pastoris was able to produce about 5400 U/ml of xylanase after 10 days of induction. With P. methanolica, on the other hand, about 6200 U/ml of xylanase was reached after 10 days of induction using synthetic medium as first culture medium and then direct induction by continuous methanol feed at 1.8 ml l−1 h−1. In general, the advantages of using P. methanolica to produce the xylanase included higher protein production, the lack of medium replacement, and an ease of scale up. However, because the P. pastoris culture supernatant contained fewer secreted non-target proteins compared to P. methanolica, xylanase purification would be easier with the P. pastoris system. In addition, experiments involving methanol pulses suggested that a relationship exists among base feeding, methanol consumption and xylanase activity.

Keywords
Pichia pastoris; Pichia methanolica; Ruminal fungi; Xylanase; High-level expression
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Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 6, 5 May 2008, Pages 459–465
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us