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Synergistic associations between Clostridium cellulovorans enzymes XynA, ManA and EngE against sugarcane bagasse

Paper ID Volume ID Publish Year Pages File Format Full-Text
17843 42700 2008 7 PDF Available
Title
Synergistic associations between Clostridium cellulovorans enzymes XynA, ManA and EngE against sugarcane bagasse
Abstract

The synergistic associations between two hemicellulases and endoglucanase E from Clostridium cellulovorans on various substrates were investigated. The recombinant enzymes were expressed, purified using affinity chromatography and tested for activity against sugarcane bagasse, xylan, carboxymethycellulose and locust bean gum. The results obtained illustrated synergistic associations between the three recombinant cellulosomal enzymes. Novel synergistic associations were established between mannanase A and endoglucanase E, as well as between xylanase A and mannanase A, in the degradation of sugarcane bagasse, xylan, carboxymethylcellulose and locust bean gum. The combination with the molar ratio of xylanase A to endoglucanase E of 75%:25% against sugarcane bagasse produced the greatest synergistic association (degree of synergy of 3.59). However, a molar ratio of xylanase A to endoglucanase E to mannanase A of 25%:25%:50% produced the largest degree of synergy against sugarcane bagasse and the mixed substrate containing 2% (w/v) birchwood xylan, 2% (w/v) carboxymethylcellulose and 2% (w/v) locust bean gum. These optimal enzyme combinations are important for the optimal release of reducing sugars from lignocellulosic waste materials such as sugarcane bagasse for subsequent biofuel production.

Keywords
Bagasse; Cellulosome; Clostridium cellulovorans; Endoglucanase E; Lignocellulose; Mannanase A; Synergy; Xylanase A
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Synergistic associations between Clostridium cellulovorans enzymes XynA, ManA and EngE against sugarcane bagasse
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 6, 5 May 2008, Pages 492–498
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us