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Periplasmic chaperone FkpA reduces extracytoplasmic stress response and improves cell-surface display on Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
17845 42700 2008 8 PDF Available
Title
Periplasmic chaperone FkpA reduces extracytoplasmic stress response and improves cell-surface display on Escherichia coli
Abstract

When enhanced yellow fluorescence protein (EYFP) was displayed on the cell surface of Escherichia coli using EstA* as a carrier, the integrity of the cell envelope was compromised and cell physiology was severely impaired, resulting in poor display performance. Coexpression of fkpA, a gene encoding periplasmic chaperone, was demonstrated to be effective for restoring cell physiology and enhancing display performance. On the basis of monitoring the promoter activities of degP, rpoH, and cpxP under various culture conditions, we also showed that cell-surface display using EstA* as a carrier induced the extracytoplasmic stress response and the stress could be partially alleviated by fkpA coexpression.

Keywords
Cell-surface display; Yellow fluorescent protein; Cell physiology; FkpA; Chaperone; Recombinant protein production; Stress regulon
First Page Preview
Periplasmic chaperone FkpA reduces extracytoplasmic stress response and improves cell-surface display on Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 6, 5 May 2008, Pages 506–513
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering