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Prospects of in vivo31P NMR method in glyphosate degradation studies in whole cell system

Paper ID Volume ID Publish Year Pages File Format Full-Text
17852 42701 2009 6 PDF Available
Title
Prospects of in vivo31P NMR method in glyphosate degradation studies in whole cell system
Abstract

The degradation of the phosphonate herbicide glyphosate (N-phosphonomethylglycine) by four taxonomically distinct microorganisms was studied in vivo in whole cell system using phosphorus nuclear magnetic spectroscopy (31P NMR). The time-course of glyphosate metabolization in dense cell cultures was followed by means of 31P NMR up to 21 days after the addition. The results obtained by this non-invasive way confirmed that the cells of Spirulina platensis and Streptomyces lusitanus biodegrade herbicide. Moreover, phosphorus starvation influenced the rate of glyphosate degradation by S. platensis. On the other hand, the results of similar measurements in the cultures of green algae Chlorella vulgaris showed that this aquatic plant, however growing in the medium containing 1 mM of N-phosphonomethylglycine, did not seem to posses the ability of its biodegradation. Additionally, the use of this method allowed us to find the new fungal strain Fusarium dimerum, which is able to biodegrade and utilize the glyphosate as the sole source of phosphorus. The results of our studies on usefulness of in vivo31P NMR for tracing glyphosate degradation in whole cell systems revealed that this non-invasive, one-step method, might be considered as a valuable tool in environmental biotechnology of organophosphonate xenobiotics.

Keywords
Biodegradation; Glyphosate; In vivo31P NMR; Xenobiotic metabolism; Spirulina platensis; Fusarium dimerum; Streptomyces lusitanus; Chlorella vulgaris
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Prospects of in vivo31P NMR method in glyphosate degradation studies in whole cell system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 1, 6 January 2009, Pages 11–16
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us