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Expression of carotenogenic genes and carotenoid production in Haematococcus pluvialis under the influence of carotenoid and fatty acid synthesis inhibitors

Paper ID Volume ID Publish Year Pages File Format Full-Text
17863 42702 2009 6 PDF Available
Title
Expression of carotenogenic genes and carotenoid production in Haematococcus pluvialis under the influence of carotenoid and fatty acid synthesis inhibitors
Abstract

Regulation of carotenogenesis in Haematococcus pluvialis was studied using various carotenoid and fatty acid synthesis inhibitors under high light in nutrient sufficient (NS/HL) and nutrient deficient (ND/HL) condition. The results showed inhibition of astaxanthin by all inhibitors studied except cerulenin in ND/HL. Total carotenoids were reduced by inhibitors except DPA in ND/HL. Lycopene accumulation was observed with nicotine treatment while β-carotene accumulation was observed with DPA. Under NS/HL condition, expression of carotenogenic gene PSY (phytoene synthase) was enhanced by all inhibitors studied except DPA while PDS (phytoene desaturase) expression was reduced by all inhibitors tested except nicotine. Expression of LCY (lycopene cyclase) was enhanced by inhibitors. Reduced expression of BKT (β-carotene ketolase) and CHY (β-carotene hydroxylase) were observed under DPA and NF treatment while enhanced expression for BKT was observed under nicotine treatment and CHY expression under nicotine and cerulenin. ND/HL exposure enhanced carotenogenic genes expression when compared to respective inhibitors of NS/HL in most cases. Reduced expression of LCY by nicotine resulted in increased lycopene accumulation while PSY and LCY by cerulenin resulted in decreased β-carotene and lutein. The results indicate the involvement of other regulatory factors besides transcriptional regulation of carotenogenesis in H. pluvialis.

Keywords
Astaxanthin; Carotenogenic genes; Haematococcus pluvialis; Cerulenin; Diphenylamine; Nicotine; Norflurazon
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Expression of carotenogenic genes and carotenoid production in Haematococcus pluvialis under the influence of carotenoid and fatty acid synthesis inhibitors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 2, 7 August 2009, Pages 88–93
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us