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Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug

Paper ID Volume ID Publish Year Pages File Format Full-Text
17866 42702 2009 6 PDF Available
Title
Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug
Abstract

Here we present a simple bioreactor with immobilized rat hepatic microsomal membranes which may be applicable in screening and prediction of reactive intermediates (RI) using alumina membrane as a support. To monitor the effectiveness of our technique, acetaminophen (AP) already known to produce RI has been utilized as a model compound. The resulting immobilized enzyme membrane (IEM) was able to produce reasonable amount of glutathione conjugates within a residence time of seconds. At the other end biosensor was tailored to predict RI by relating the enhanced activities of microsomal glutathione S-transferase (mGST) as a marker of drug and chemical stress.

Keywords
Microsome; mGST; P450; Microreactor; Reactive intermediate; Biosensor; Immobilization
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Screening and prediction of reactive intermediates in a microreactor with immobilized rat hepatic microsomes using acetaminophen as a model drug
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 2, 7 August 2009, Pages 112–117
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us