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A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
17869 42702 2009 5 PDF Available
Title
A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures
Abstract

During microbial bioprocesses development, it is crucial monitoring cell stress response as such information determines the process efficiency.Multi-parameter flow cytometry coupled with fluorescent stains [PI and DiOC6(3) (3,3′-dihexylocarbocyanine iodide)] was used to monitor bacterial batch cultures. It was observed that the most significant differences in the cell physiological responses of these microorganisms were observed at the early stationary phase. While Bacillus licheniformis batch culture displayed 91% of healthy cells, 1.3% of cells with depolarised cytoplasmic membrane and 8% of cells with both depolarised and permeabilised cytoplasmic membrane as the culture reached this phase, Lactobacillus rhamnosus batch culture displayed 66% of healthy cells, 29% of cells with depolarised cytoplasmic membrane and 5% of depolarised and permeabilised cytoplasmic membrane, at that stage. However, at the late stationary phase, both bacterial cultures showed a high proportion of polarised healthy cells, suggesting that the microorganisms could be using storage materials as a survival strategy.It was found that the double staining protocol using PI and DiOC6(3) could be used to monitor cell physiological response of both bacterial batch cultures and provided important physiological information at the individual cell level that is difficult to obtain in any other way, which can be used to enhance bioprocesses efficiency.

Keywords
Lactobacillus rhamnosus; Bacillus licheniformis; Cell physiological response; PI; DiOC6(3); Flow cytometry
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A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 45, Issue 2, 7 August 2009, Pages 134–138
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us