fulltext.study @t Gmail

A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device

Paper ID Volume ID Publish Year Pages File Format Full-Text
17884 42703 2008 6 PDF Available
Title
A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device
Abstract

A novel on-line monitoring approach implementing an improved sampling methodology is presented here. A sample is directly withdrawn from the bioreactor without a need for a filtration or a dialysis probe, extensively diluted in a flow-through fashion and mixed with a mobile phase consisting of a buffer and a soluble mediator. The developed sampling system efficiently controls dilution of the sample prior to biosensor assay covering full dynamic range of glycerol concentrations during its fermentation to 1,3-propanediol. The new sampling protocol minimizes problems associated with the use of traditional on-line sampling methods implementing membrane separation or filtration techniques. Concentration of glycerol in the sample is determined by a flow injection analysis (FIA) with an amperometric biosensor using glycerol dehydrogenase and diaphorase with a throughput of nine samples per hour. The biosensor performance was validated by a reference analytical method (HPLC) with a very good agreement.

Keywords
Biosensor; On-line bioprocess monitoring; Probe-free sampling; Glycerol; 1,3-Propanediol; Biodiesel
First Page Preview
A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 5, 4 April 2008, Pages 434–439
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us