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Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads

Paper ID Volume ID Publish Year Pages File Format Full-Text
17894 42704 2007 8 PDF Available
Title
Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads
Abstract

In this study, chitosan beads (Ch-bead) and cibacron blue F3GA attached chitosan beads (CB-Ch-bead) were prepared. Their characteristics were investigated with experiments of swelling, thermogravimetric analysis and Fourier transform infrared (FTIR) spectroscopic analysis. Catalase (CAT) was immobilized onto these beads. The adsorption isotherms have a Langmuirian shape for Ch-beads and CB-Ch-beads. The CAT adsorption capacity of Ch-beads is higher than that of CB-Ch-beads, but CB-Ch-CAT showed better activity according to the Ch-CAT. The values of apparent Km were found to be 18 and 41 mM for Ch-CAT and CB-Ch-CAT, respectively. However, Vmax values were calculated as 4800 and 14,250 μmol (mg protein)−1 min−1 for Ch-CAT and CB-Ch-CAT, respectively. Furthermore, various characteristics of immobilized catalase, such as the temperature profile, thermal stability, optimum pH, operational stability and storage stability were evaluated. Optimum temperature and optimum pH values were found as 35 °C and 7.0 for maximum activity of Ch-CAT and CB-Ch-CAT. It was observed that thermal, storage and operational stabilities of the enzyme were increased with immobilization.

Keywords
Chitosan beads; Cibacron blue F3GA; Catalase; Adsorption; Immobilization
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Immobilization of catalase onto chitosan and cibacron blue F3GA attached chitosan beads
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 447–454
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us