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Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
17899 42704 2007 8 PDF Available
Title
Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation
Abstract

In this work, a thermostable laccase from the fungus Melanocarpus albomyces was produced in the efficient protein producer Trichoderma reesei in batch, fed-batch and continuous cultivations on lactose medium in a laboratory fermenter. The rate of consumption of base for pH control (DELTABAS) was measured in all the cultivations and was used as a control parameter in one fed-batch run and in a 1000 h continuous cultivation.The dynamic DELTABAS control parameter can be used to maintain optimal production of secreted protein by fitting the rate of substrate feeding to the amount of substrate available to the growing fungus in the fermenter, thus maintaining growth limitation due to availability of carbon source while avoiding total carbon source depletion. The use of this parameter, capable of maintaining the biomass in a physiological state of imminent but not actual exhaustion of carbon source, was successful in both the fed-batch and continuous production of heterologous laccase.Laccase production in fed-batch and continuous runs was considerably improved in comparison to the batch cultivation, both in terms of volumetric productivity and yield on lactose. The DELTABAS-control algorithm was shown to be a useful research tool for process optimization.

Keywords
Trichoderma reesei; Laccase production; Fed-batch; Continuous; Fermentation
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Use of a growth-associated control algorithm for efficient production of a heterologous laccase in Trichoderma reesei in fed-batch and continuous cultivation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 484–491
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us