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Allosteric molecular sensing of anti-HIV antibodies by an immobilized engineered β-galactosidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
17900 42704 2007 6 PDF Available
Title
Allosteric molecular sensing of anti-HIV antibodies by an immobilized engineered β-galactosidase
Abstract

The main objective is to demonstrate the feasibility of qualitative and semi-quantitative determination of anti-HIV antibodies, based on the allosteric response of an immobilized engineered Escherichia coli β-galactosidase. The suitability of this enzyme and the conditions for homogeneous assay had been previously demonstrated. In the present work, the operational conditions for engineered E. coli β-galactosidase immobilization on activated agarose supports have been determined. A solid biocatalyst has been prepared and characterized in order to determine the activation degree of the immobilized enzyme in the presence of anti-HIV antibodies. The solid-phase biocatalyst has been shown to be able to modulate its activity and produce an unequivocal signal in presence of anti-HIV containing sera from different patients. Finally, the conceptual design of a possible assay based on the above methodology is proposed.

Keywords
Immobilized engineered β-galactosidase; Anti-HIV antibodies sensing; Allosteric enzymes
First Page Preview
Allosteric molecular sensing of anti-HIV antibodies by an immobilized engineered β-galactosidase
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 492–497
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering