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Activation of nylon net and its application to a biosensor for determination of glucose in human serum

Paper ID Volume ID Publish Year Pages File Format Full-Text
17911 42705 2009 5 PDF Available
Title
Activation of nylon net and its application to a biosensor for determination of glucose in human serum
Abstract

A glucose biosensor using a glucose oxidase (GOx)-immobilized nylon net with glutaraldehyde as cross-linking reagent and an oxygen (O2) electrode for the determination of glucose has been fabricated. The detection scheme was based on the utilization of dissolved O2 in oxidation of glucose by the membrane bound GOx. Crucial factors including O-alkylation temperature, reaction times of nylon net with dimethyl sulfate, l-lysine, and glutaraldehyde, and enzyme loading were examined to determine the optimal enzyme immobilization conditions for the best sensitivity of the developed glucose biosensor. In addition, the effects of pH and concentration of phosphate buffer on the response of the biosensor were studied. The glucose biosensor had a linear range of 18 μM to 1.10 mM with the detection limit of 9.0 μM (S/N = 3) and response time of 80 s. The biosensor exhibited both good operational stability with over 200 measurements and long-term storage stability. The results from this biosensor compared well with those of a commercial glucose assay kit in analyzing human serum glucose samples.

Keywords
Glucose biosensor; Glucose oxidase; Nylon net; Activation; Glucose
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Activation of nylon net and its application to a biosensor for determination of glucose in human serum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 5, 6 May 2009, Pages 249–253
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us