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Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
17914 42705 2009 5 PDF Available
Title
Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae
Abstract

Adenosine triphosphate (ATP) is necessary in the enzymatic production of glutathione (GSH). Our aim was to improve GSH production by increasing the efficiency of ATP regeneration in a coupled system. Previous results suggested that low GSH production in the coupled system is due to the irreversible transformation of adenosine (Ado) to hypoxanthine (Hx) via inosine (Ino) pathway in Escherichia coli JM109 (pBV03). In this study, to block the transformation of Ado into Hx and enhance GSH production, a coupled ATP regeneration system was constructed with adenosine deaminase-deficient recombinant E. coli JW1615 (pBV03) and Saccharomyces cerevisiae WSH2. GSH production was improved (2.94-fold of the control), and ATP regeneration reaction was established in the coupled system. The results are applicable to ATP regeneration in other microbial processes.

Keywords
Adenosine deaminase; Adenine deaminase; ATP; Escherichia coli; Glutathione production; Saccharomyces cerevisiae
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Enhancement of glutathione production in a coupled system of adenosine deaminase-deficient recombinant Escherichia coli and Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 44, Issue 5, 6 May 2009, Pages 269–273
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us