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Development of a promoter assay system for the flavinogenic yeast Candida famata based on the Kluyveromyces lactis β-galactosidase LAC4 reporter gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
17931 42706 2008 8 PDF Available
Title
Development of a promoter assay system for the flavinogenic yeast Candida famata based on the Kluyveromyces lactis β-galactosidase LAC4 reporter gene
Abstract

A system for analysis of promoter activities was developed for the flavinogenic yeast Candida famata. The Kluyveromyces lactis LAC4 gene encoding β-galactosidase as a reporter gene and C. famata mutant lac4 unable for lactose utilization as a recipient strain were used in the system. The Escherichia coli β-galactosidase gene lacZ could not be used in the system because of difference in codon usage between the bacterium and C. famata. The C. famata mutant lac4 was transformed with the plasmid containing analyzable promoters fused with the promoterless LAC4 gene. Resulting transformants (unlike the mutant lac4) were able to utilize lactose as sole carbon source. The promoter strength was estimated on the basis of β-galactosidase activity assayed in the transformants. Different promoters of C. famata and Debaryomyces hansenii (a teleomorph of C. famata) were analyzed using this approach. The results showed an adequacy of the K. lactis LAC4 gene for evaluation of promoter strength in C. famata.

Keywords
LAC4; Yeast β-galactosidase; Reporter gene; Yeast promoters; Promoter strength; Candida famata
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Development of a promoter assay system for the flavinogenic yeast Candida famata based on the Kluyveromyces lactis β-galactosidase LAC4 reporter gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 3, 4 February 2008, Pages 208–215
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us