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Characterization of chitosan and chitosan–glucan complex extracted from the cell wall of fungus Gongronella butleri USDB 0201 by enzymatic method

Paper ID Volume ID Publish Year Pages File Format Full-Text
17936 42706 2008 10 PDF Available
Title
Characterization of chitosan and chitosan–glucan complex extracted from the cell wall of fungus Gongronella butleri USDB 0201 by enzymatic method
Abstract

The fungus Gongronella butleri USDB 0201 was grown on sweet potato pieces supplemented with mineral and urea solution. Mycelia were harvested after 7 days of cultivation. Chitosan in the fungal cell wall exists in two forms, free chitosan and chitosan bounded to β-glucan. The linkage between chitosan and β-glucan in the chitosan–glucan complex (CGC) was successfully cleaved using a heat stable α-amylase. The resultant chitosan and β-glucan polymeric moieties were characterized. Data from elementary analysis, IR and 13C NMR spectroscopy confirmed that all intramolecular glycosidic linkages in either chitosan or glucan polymeric moieties are in the β-configuration. Data from specific enzymatic degradation of the CGC obtained from different treatments and reducing sugar analysis indicate that chitosan and glucan are linked by an intermolecular α-1,4 glycosidic bond. These evidences supported to prove the β-linked chitosan and β-linked glucan are linked with α-1,4 glycosidic bond. However heat stable α-amylase enzyme could not cleave the CGC completely. Therefore high yield with pure chitosan could be obtained from the cell wall of fungus G. butleri USDB 0201 by Termamyl enzyme treatment.

Keywords
Chitin/chitosan; Chitosan–glucan complex (CGC); Fungal cell wall; Glucan; Glycosidic bond
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Characterization of chitosan and chitosan–glucan complex extracted from the cell wall of fungus Gongronella butleri USDB 0201 by enzymatic method
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 3, 4 February 2008, Pages 242–251
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us