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Bicontinuous microemulsion as reaction medium for the β-glucosidase-catalyzed synthesis of n-hexyl-β-d-glucopyranoside

Paper ID Volume ID Publish Year Pages File Format Full-Text
17937 42706 2008 7 PDF Available
Title
Bicontinuous microemulsion as reaction medium for the β-glucosidase-catalyzed synthesis of n-hexyl-β-d-glucopyranoside
Abstract

n-Hexyl-β-d-glucopyranoside (HGP) was synthesized through the β-glucosidase-catalyzed condensation of glucose and hexanol in an aqueous-organic bicontinuous microemulsion system with both water and oil as continuous phases. Methodology for separation of HGP from the microemulsion system for analysis by gas chromatography was developed. Various factors influencing the synthesis of HGP like reaction time, enzyme concentration and temperature were optimized experimentally. β-Glucosidase (1.1 U/ml) yielded a final HGP concentration of 6.5 mM at 45 °C after 24 h reaction. Reverse reaction (hydrolysis) occurred was also studied. Comparison of the rate of the reaction between synthesis and hydrolysis showed the latter to be faster, suggesting that bicontinuous microemulsion system is more applicable to the enzymatic hydrolysis. Degradation of enzyme in bicontinuous microemulsion system was very negligible.

Keywords
Bicontinuous microemulsion; n-Hexyl-β-d-glucopyranoside; β-Glucosidase; Enzymatic synthesis; Enzymatic hydrolysis
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Bicontinuous microemulsion as reaction medium for the β-glucosidase-catalyzed synthesis of n-hexyl-β-d-glucopyranoside
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 3, 4 February 2008, Pages 252–258
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us