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Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy

Paper ID Volume ID Publish Year Pages File Format Full-Text
17939 42706 2008 7 PDF Available
Title
Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy
Abstract

A very stable α-galactosidase from Thermus sp. T2 has been immobilized on different supports activated with glyoxyl, epoxy or glutaraldehyde groups. Although all preparations retained very high activity (usually over 90%) and all immobilization protocols improved the enzyme stability, the best stability was obtained by immobilization on glutaraldehyde activated supports. Using glutaraldehyde, we compared the immobilization of the enzyme on pre-activated supports or the modification with glutaraldehyde of the enzyme previously adsorbed on amino-supports. The last strategy gave even more stable preparations, retaining over 90% of initial activity. Optimal conditions for the preparation of the immobilized preparations were 1% (v/v) glutaraldehyde and support activated with 40 μmol/mL of support. This preparation retained 90% initial activity after 48 h at pH 7 and 75 °C while the soluble enzyme was fully inactivated after only 8 h. Moreover, this immobilization protocol improved the optimal temperature from 65 °C (soluble enzyme) to 70 °C.

Keywords
Enzyme immobilization; Enzyme stabilization; Thermophilic enzymes; Soy milk; Glutaraldehyde
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Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 3, 4 February 2008, Pages 265–271
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us