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Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S. cerevisiae via expression of β-glucosidase on its cell surface

Paper ID Volume ID Publish Year Pages File Format Full-Text
17945 42707 2008 4 PDF Available
Title
Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S. cerevisiae via expression of β-glucosidase on its cell surface
Abstract

Co-utilization of several sugars, especially xylose and glucose, is essential for economically feasible processes with high ethanol productivity. However, the major problem during xylose/glucose co-fermentation is that xylose is used very slowly until after glucose is completely consumed. Here, we demonstrated an effective co-fermentation process using xylose- and cellobiose-assimilating recombinant Saccharomyces cerevisiae. The recombinant yeast is able to consume xylose during xylose/cellobiose co-fermentation as rapidly as during glucose fermentation. After 72 h, 95.6% of xylose was consumed, despite the co-fermentation conditions, and the ethanol yield was 0.358 g-ethanol/g-total sugar.

Keywords
Saccharomyces cerevisiae; Cell surface display; Xylose fermentation; Co-fermentation; β-Glucosidase
First Page Preview
Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S. cerevisiae via expression of β-glucosidase on its cell surface
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 3, 5 September 2008, Pages 233–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering