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A microbial biosensing system for monitoring methane

Paper ID Volume ID Publish Year Pages File Format Full-Text
17949 42707 2008 5 PDF Available
Title
A microbial biosensing system for monitoring methane
Abstract

A microbial biosensing system for the measurement of methane has been developed using an immobilized mixed culture of Pseudomonas aeruginosa and Klebsiella sp. together with a dissolved oxygen (O2) sensor. When methane was applied to the biosensing system, the dissolved O2 content decreased until a steady-state was reached. The biosensing system response depends linearly on methane concentration between 1.0 and 5.0% (v/v) with a detection limit of 0.3% (v/v) (S/N = 3) and a 100-s response time. Phosphate buffer (pH 7.0, 25 mM) and room temperature (20–25 °C) were chosen as the optimum working conditions. Some parameters including pH, temperature, operational and storage stability were studied in detail for characterization of the biosensing system.

Keywords
Methane; Microbial biosensing; Dissolved oxygen sensor
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A microbial biosensing system for monitoring methane
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 3, 5 September 2008, Pages 257–261
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us