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Effect of furfural on nitrogen assimilating enzymes of the lactose utilizing yeasts Candida blankii 35 and Candida pseudotropicalis 11

Paper ID Volume ID Publish Year Pages File Format Full-Text
17953 42707 2008 5 PDF Available
Title
Effect of furfural on nitrogen assimilating enzymes of the lactose utilizing yeasts Candida blankii 35 and Candida pseudotropicalis 11
Abstract

The research was performed with two lactose-assimilating yeast strains—Candida blankii 35 with an oxidative metabolism and Candida pseudotropicalis 11 with a fermentative metabolism. The strains were cultivated in a chemostat under carbon limitation at dilution rates D = 0.1 and 0.25 h−1. After shock with 0.04% furfural, the changes of the following nitrogen enzyme activities were studied: NADPH-dependant glutamate dehydrogenase (NADPH-GDH), NADH-dependant glutamate dehydrogenase (NADH-GDH), glutamine synthetase (GS), glutamate synthase (GOGAT) and alanine dehydrogenase (ADH).Results show that during cultivation of C. blankii 35 at D = 0.1 h−1, furfural exhibits a clear inhibitory effect on the glutamate dehydrogenase and the alanine metabolite routes of ammonia ion assimilation. Furfural slows down the nitrogen assimilation of the strain through glutamate dehydrogenase and at D = 0.1 h−1 the GS/GOGAT system is activated.During the growth of the fermenting strain C. pseudotropicalis 11, furfural (0.04%) did not exhibit stable inhibitory effect on the enzyme activities of NADPH-GDH, NADH-GDH, GS, GOGAT and ADH. To the opposite, when reaching the steady state after furfural addition, their activities increased 6.3, 4.9, 5.6 and 4.1 times, respectively. At D = 0.25 h−1, GS activity was stronger affected by the dilution rate. Furfural did not inhibit the activity of NADPH-GDH, NADH-GDH and ADH. However, continuous inhibitory effect on GS and GOGAT activity was observed.

Keywords
Candida blankii; Candida pseudotropicalis; Furfural; NADPH- and NADH-glutamate dehydrogenase; Glutamine synthetase; Glutamate synthase; Alanine dehydrogenase
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Effect of furfural on nitrogen assimilating enzymes of the lactose utilizing yeasts Candida blankii 35 and Candida pseudotropicalis 11
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 3, 5 September 2008, Pages 284–288
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us