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Enzymatic hydrolysis of penicillin G to 6-aminopenicillanic acid in cloud point system with discrete countercurrent experiment

Paper ID Volume ID Publish Year Pages File Format Full-Text
17977 42708 2007 6 PDF Available
Title
Enzymatic hydrolysis of penicillin G to 6-aminopenicillanic acid in cloud point system with discrete countercurrent experiment
Abstract

A set of flasks was utilized to simulate a discrete countercurrent bioreactor. An enzymatic hydrolysis of penicillin G to 6-aminopenicillanic acid with immobilized penicillin acylase in a cloud point system was carried out with the discrete countercurrent operational mode. Byproduct phenylacetic acid is effectively transported with the auxiliary phase (coacervate phase), while product 6-aminopenicillanic acid and reactant penicillin G are effectively transported with the aqueous phase, where the enzymatic hydrolysis of penicillin G takes place. The experimentally results show that the product concentration and product yield are improved from the batch operational mode of about 38 mM and 50% to the corresponding countercurrent operational mode of above 50 mM and 65%, respectively. However, experimentally examination of the effect of operational parameters, such as feed point location, volume ratio of auxiliary phase to aqueous phase and initial reactant concentration, indicates that the achievement of a high product concentration and a high product yield at the same time is difficult by a simple utilization of the continuous countercurrent operational mode. This can be contributed to the facts that the enzymatic hydrolysis favors at a relatively higher pH, but the extraction of PAA only takes place at a relatively lower pH.

Keywords
Countercurrent bioreactor; Cloud point system; Enzymatic hydrolysis; Penicillin G
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Enzymatic hydrolysis of penicillin G to 6-aminopenicillanic acid in cloud point system with discrete countercurrent experiment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issues 1–2, 2 July 2007, Pages 121–126
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us