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Quantum dot-based pH probe for quick study of enzyme reaction kinetics

Paper ID Volume ID Publish Year Pages File Format Full-Text
17978 42708 2007 6 PDF Available
Title
Quantum dot-based pH probe for quick study of enzyme reaction kinetics
Abstract

Water-soluble luminescent CdTe–ZnS quantum dots (QDs) modified by mercaptopropyl-acid was used to determinate H+ in aqueous solutions. The fluorescence (FL) of the water-soluble QDs could be irreversibly quenched by H+ and the fluorescence intensity of the water-soluble QDs decreased linearly as the pH decreased in the range of 8.0–5.0. Based on this phenomenon, a simple, rapid and specific method to quick determination of enzyme reaction kinetics was proposed. The modified QDs was successfully used as pH probe in monitoring the hydrolysis of glycidyl butyrate catalyzed by porcine pancreatic lipase (PPL). The proposed method demonstrated improved sensitivity, stability and a wider monitoring range for H+ determinations as compared to the already described analytical methods based on PNP.

Keywords
Quantum dot; Fluorescence intensity; pH probe; Quench; Enzyme; Hydrolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issues 1–2, 2 July 2007, Pages 127–132
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us