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Production of gastrodin through biotransformation of p-2-hydroxybenzyl alcohol by cultured cells of Armillaria luteo-virens Sacc

Paper ID Volume ID Publish Year Pages File Format Full-Text
17992 42709 2008 6 PDF Available
Title
Production of gastrodin through biotransformation of p-2-hydroxybenzyl alcohol by cultured cells of Armillaria luteo-virens Sacc
Abstract

Gastrodin is the important ingredients of Gastrodia elata Blume owing to its important pharmacological properties. The aim of the current research is to introduce one-step microbial bioconversion of p-2-hydroxybenzyl alcohol (HBA) to gastrodin instead of chemical synthesis. The method of gastrodin measurement was firstly constructed. Structural elucidation of biotransformed compounds was based on one-dimensional and two-dimensional NMR. Nine resting-filamentous cells suspension are screened for their transforming capabilities to produce gastrodin. The microorganisms used were Armillaria luteo-virens Sacc (CGMCC no. 1884), Aspergillus foetidus ZU-G1 (CGMCC no. 1628), Peniciuium cyclopium AS 3.4513, Aspergillus niger AS 3.40, A. niger AS 3.429, Trichoderma viride AS 3.4005, Trichoderma sp., Penicillium notatum, Mucor sp., Rhizopus sp. A. luteo-virens Sacc U gave the highest gastrodin concentration. The optimized bioconversion conditions for A. luteo-virens Sacc U consisted of inoculums size 160.0 ± 10.5 g resting cells/L, amounts of precursor (HBA) 10.0 mg/ml, bioconversion system pH 6.0, 1% (v/v) Tween 80 or 0.1% oleic acid, bioconversion temperature 23 °C, shaking speed 120 rpm in darkness. Under the optimized transformation conditions, the highest gastrodin concentration was 750 ± 38 μg/100 ml at 5 days. The results of current study cannot only apply to replace the chemical synthesis process that involves the glycosylation reaction, but also apply to scale-up production.

Keywords
Filamentous fungi; Gastrodin; HBA; Bioconversion condition; Armillaria luteo-virens Sacc
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Production of gastrodin through biotransformation of p-2-hydroxybenzyl alcohol by cultured cells of Armillaria luteo-virens Sacc
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 1, 7 July 2008, Pages 25–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us