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Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers

Paper ID Volume ID Publish Year Pages File Format Full-Text
17993 42709 2008 4 PDF Available
Title
Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers
Abstract

Novel strain development strategies were used to produce secreted recombinant human gelatin in Pichia pastoris. Strains were first constructed using antibiotic resistance markers to select for multicopy strains. To determine if use of an additional expression cassette with another antibiotic marker could further increase copy number, the initial set of strains were either re-electroporated or mated with a plasmid or strain containing a different marker. Crossing strains expressing 25 and 50 kDa gelatin resulted in progeny that expressed both gelatins, demonstrating that a genetic strategy may successfully combine expression cassettes. Quantitative evaluation of strains produced by either single or double marker strategies demonstrated that increased expression cassette copy number and gelatin expression levels were obtained by both methods using two antibiotic resistance markers.

Keywords
Multicopy strains; Pichia pastoris; Recombinant gelatin
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Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 1, 7 July 2008, Pages 31–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us