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Purification and characterization of a novel glycosidase from the china white jade snail (Achatina fulica) showing transglycosylation activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
17994 42709 2008 8 PDF Available
Title
Purification and characterization of a novel glycosidase from the china white jade snail (Achatina fulica) showing transglycosylation activity
Abstract

A broad specificity β-d-glycosidase, designated G I, was purified to homogeneity from the viscera of the China white jade snail (Achatina fulica). The enzyme was a monomeric protein with molecular weight of 115 kDa. G I has broad glycone specificity towards p-nitrophenyl derivatives of β-d-glucose, β-d-fucose, β-d-galactose, α-d-glucose and some disaccharides. The optimum pH and temperature are 5.5 and 55 °C, respectively. It was stable over a wide pH range (5–10 at 30 °C for 24 h) and against a relatively high temperature (50 °C for 4 h). Moreover, it was also stable and active in the presence of various alcohols. With pNPGlu, pNPFuc and cellobiose as donor, G I showed high transglycosylation activity. Six transglycosylation products were isolated from the reaction mixture containing 20% alcohol as glycoside acceptor using a preparative thin layer chromatography (preparative TLC) and identified as alkyl-glucosides and alkyl-fucosides by mass spectrometry (MS) analysis. Combining the high alcohol tolerance, moderate temperature and pH stability and alkyl glycosides production efficiency through transglycosylation, G I can be considered to be a promising candidate for the production of various alkyl glycosides.

Keywords
β-d-Glycosidase; Transglycosylation; Thermal and pH stability; Broad specificity; Alkyl glycosides; Enzyme purification
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Purification and characterization of a novel glycosidase from the china white jade snail (Achatina fulica) showing transglycosylation activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 1, 7 July 2008, Pages 35–42
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us