fulltext.study @t Gmail

Trichoderma atroviride mutants with enhanced production of cellulase and β-glucosidase on pretreated willow

Paper ID Volume ID Publish Year Pages File Format Full-Text
17996 42709 2008 8 PDF Available
Title
Trichoderma atroviride mutants with enhanced production of cellulase and β-glucosidase on pretreated willow
Abstract

Trichoderma atroviride mutants were developed by using N-methyl-N′-nitro-N-nitrosoguanidine (NTG) treatment and UV-light followed by a semiquantitative plate clearing assay on Walseth-cellulose/agar plates. The parent strain of the mutants was an isolate from the Amazonas basin (TUB F-1505), whose identity was established by ITS 1 and 2 and tef1 gene sequence analysis. Strain F-1505 proved to be the most promising extracellular cellulase producer among 150 wild-type Trichoderma in a screening program performed in shake flask fermentation on pretreated willow. Reducing sugar content, soluble protein, filter paper cellulase activity (FPA), β-glucosidase activity and endoglucanase activity of the fermentation broths of the mutant strains were measured in both shake flask and lab-scale fermenters and compared with Trichoderma reesei Rut C30. Also, hydrolytic capacities of fermentation supernatants of T. reesei Rut C30, the parent strain (F-1505) and the best mutants were compared on pretreated willow as carbon source and hydrolysis substrate. The T. atroviride mutants produced high levels of extracellular cellulases as well as β-glucosidase, rendering the need for β-glucosidase supplementation in hydrolysis of cellulose or pretreated willow unnecessary. On the contrary, β-glucosidase supplementations were essential in order to obtain good glucose yields for all other cellulase preparations tested.

Keywords
Trichoderma atroviride; Cellulase production; β-Glucosidase production; Pretreated willow; Fermentation; Enzymatic hydrolysis of cellulose
First Page Preview
Trichoderma atroviride mutants with enhanced production of cellulase and β-glucosidase on pretreated willow
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 43, Issue 1, 7 July 2008, Pages 48–55
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us