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Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii

Paper ID Volume ID Publish Year Pages File Format Full-Text
18007 42710 2007 7 PDF Available
Title
Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii
Abstract

Different immobilization strategies have been issued to prepare a stable and active biocatalyst of the formate dehydrogenase from Candida boidinii: immobilization on glutaraldehyde, epoxy, amino-epoxy, glyoxyl or treatment of enzymes adsorbed on aminated supports with glutaraldehyde. The best results in terms of stability were achieved using amino-epoxy supports (by a 12-fold factor compared to soluble and BrCN immobilized enzyme) and glyoxyl agarose supports (by a 150-fold factor), although in both cases activity recovery was just over 15%. The use of milder conditions permitted to improve the recovered activity, but decreased in a parallel way the enzyme stability, until being almost identical to the soluble enzyme.Alternatively, ionic adsorption of the enzyme was assayed. Supports coated with PEI permitted to keep full activity of the enzyme, permitted a very strong adsorption and the reutilization of the support after enzyme inactivation. Moreover, all the immobilization techniques prevent enzyme inactivation under strong stirring, necessary to keep the pH value and eliminate the CO2 formed during the reaction.In this way, a battery of solutions to immobilize this enzyme has been proposed, fulfilling the different requirements that the use of this NADH recycling enzyme may have, depending on the particular reaction.

Keywords
CNBr, cyanogen bromide; EC-HFA, epoxy-EDA-sepabeads supports; EDA, ethylenediamine; FDH, formate dehydrogenase; MANAE, monoaminoethyl-N-ethy; NAD+, nicotinamide adenine dinucleotide; PEI, polyethylenimineFormate dehydrogenase; Enzyme stabilization; Amino-
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Evaluation of different immobilization strategies to prepare an industrial biocatalyst of formate dehydrogenase from Candida boidinii
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 540–546
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us