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Biotransformation of puerarin into 3′-hydroxypuerarin by Trichoderma harzianum NJ01

Paper ID Volume ID Publish Year Pages File Format Full-Text
18014 42710 2007 4 PDF Available
Title
Biotransformation of puerarin into 3′-hydroxypuerarin by Trichoderma harzianum NJ01
Abstract

Biotransformation of puerarin catalyzed by filamentous fungi has been studied. A primary screening was carried out using 138 filamentous fungi isolated from soil, that was able to grow in the presence of puerarin. Strain NJ01 was identified as Trichoderma harzianum. Under the optimum conditions, resting mycelium of T. harzianum NJ01 converted 41% of added 0.6 g/l puerarin to 3′-hydroxypuerarin that was characterized by MS, 13C NMR and 1H NMR spectra. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging system, the activity of 3′-hydroxypuerarin (3′-OHP) was 20 times higher than that of puerarin. The solubility of 3′-OHP was 1.3 times greater than that of puerarin.

Keywords
Trichoderma harzianum; Biotransformation; Puerarin; 3′-Hydroxypuerarin; Antioxidant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 594–597
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us