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Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavescens

Paper ID Volume ID Publish Year Pages File Format Full-Text
18019 42710 2007 8 PDF Available
Title
Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavescens
Abstract

Effect of different environmental and nutritional factors on succinic acid production by batch fermentation of Enterococcus flavescens and enzymes involved in the acid production through reverse tricarboxylic acid cycle (TCA) cycle was investigated. An overall seven-fold increase in succinic acid production (from 0.92 g l−1 in 72 h initially to 6.7 g l−1 in 48 h) was achieved in 300-ml of optimized medium having 3% sucrose, 1:0.5 ratio of tryptone and ammonium hydrogen phosphate, 15 mM MgCO3 at pH 6.5 when inoculated with 4% (v/v) of seed inoculum and incubated at 39 °C for 48 h, as against initial un-optimized medium. Subsequent scale-up in a 10-l bioreactor using these optimized fermentation conditions under controlled pH and continuous CO2 supply resulted in 14.25 g l−1 of succinic acid in 30 h. Optimization of the environmental and nutritional parameters resulted in a maximum production of succinic acid by affecting the levels of the enzymes involved in its production via the reverse TCA cycle. A linear relationship was observed between succinic acid production and in the enzyme activities. The enzyme activity was found to be increase in order phosphoenol pyruvate carboxykinase < malate dehydrogenase < fumarase < fumarate reductase < phosphoenol pyruvate carboxylase.

Keywords
Succinic acid; Fermentation; Anaerobic production; Enterococcus flavescens; Scale-up; Reverse TCA cycle enzymes
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Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavescens
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 629–636
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us