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Enzyme production and chemical alterations of Eucalyptus grandis wood during biodegradation by Ceriporiopsis subvermispora in cultures supplemented with Mn2+, corn steep liquor and glucose

Paper ID Volume ID Publish Year Pages File Format Full-Text
18021 42710 2007 8 PDF Available
Title
Enzyme production and chemical alterations of Eucalyptus grandis wood during biodegradation by Ceriporiopsis subvermispora in cultures supplemented with Mn2+, corn steep liquor and glucose
Abstract

Eucalyptus grandis biodegradation by Ceriporiopsis subvermispora in solid-state cultures supplemented with corn steep liquor (CSL), glucose and Mn2+ was evaluated. Chemical composition of biotreated wood was determined to correlate the extent of wood transformation with the levels of extracellular agents produced during fungal growth. Manganese-dependent peroxidases and xylanases were detected under all culture conditions. The lowest and the highest MnP activities were observed in cultures supplemented with Mn2+ and CSL/glucose (178 and 1317 IU kg−1 of dry wood, respectively). In all cultures, glucan and xylan losses were lower than 3% while lignin loss varied from 2.7% to 9%. Lignin loss was correlated with the cleavage of aryl-ether linkages detected with basis on derivatization followed by reductive cleavage (DFRC) analysis. The highest lignin loss and the lowest yield of DFRC monomers were observed in cultures supplemented with Mn2+ and CSL. This culture condition presented intermediate levels of MnP production. Oxalate production was not strongly affected by addition of culture supplements suggesting that oxalate was not a limiting agent during wood degradation by C. subvermispora. Conversely, the results suggest that a compromise between the levels of MnP and Mn2+ was necessary to promote intense degradation of lignin revealed by DFRC analysis and lignin mass balances.

Keywords
Biopulping; Ceriporiopsis subvermispora; Wood biodegradation; White-rot fungi; Lignin degradation
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Enzyme production and chemical alterations of Eucalyptus grandis wood during biodegradation by Ceriporiopsis subvermispora in cultures supplemented with Mn2+, corn steep liquor and glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 645–652
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us