fulltext.study @t Gmail

Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst

Paper ID Volume ID Publish Year Pages File Format Full-Text
18029 42710 2007 4 PDF Available
Title
Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst
Abstract

A new lipase from porcine pancreas was adsorbed on PEI-coated support and utilized in the resolution of (±)-glycidyl butyrate, reaching a moderate E value of 6. The treatment of the adsorbed lipase with glutaraldehyde permitted to increase this value by a 10-fold factor (to E = 61). Similar improvement of enantioselectivity promoted by the glutaraldehyde treatment was obtained employing immobilized preparations of other lipases. Moreover, enzyme activity in some instances can be even improved by this treatment, and the effect of the experimental conditions on the enzyme activity was completely altered. Thus, this simple treatment, in many cases necessary to stabilize the enzyme preparations, may be also a powerful tool to alter the enzyme properties.

Keywords
Crosslinking; Glutaraldehyde; Lipase modulation; Lipase immobilization; Enantioselectivity
First Page Preview
Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 704–707
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us