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Immobilization of dextransucrase on regenerated benzoyl cellulose carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
18037 42710 2007 6 PDF Available
Title
Immobilization of dextransucrase on regenerated benzoyl cellulose carriers
Abstract

The regeneration of the benzoyl cellulose carrier was accomplished by treatment by Triton X-100, sodium dodecyl sulfate (SDS), or alkyldimethylbenzyl-ammonium chloride (ADMB) detergent solutions, or by NH4SCN as chaotropic agent. It was found that Triton X-100, as a more hydrophobic substance, was linked to the carrier, eluting the protein from the carrier. Regenerated in this way, the carrier very efficiently binds new amounts of proteins, including dextransucrase, without regarding the fact that Triton X-100 was washed out by alcohol, or remained linked to the carrier. Dextransucrase immobilization at this regenerated carriers yielded high values for the immobilized activity, total preserved activity and immobilization efficiency.

Keywords
Dextransucrase; Benzoyl cellulose; Enzyme immobilization; Carrier regeneration
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Immobilization of dextransucrase on regenerated benzoyl cellulose carriers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 763–768
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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