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Chitosan-immobilized lipases for the catalysis of fatty acid esterifications

Paper ID Volume ID Publish Year Pages File Format Full-Text
18038 42710 2007 9 PDF Available
Title
Chitosan-immobilized lipases for the catalysis of fatty acid esterifications
Abstract

Lipases from Candida rugosa, Pseudomonas fluorescens and Candida antarctica B were immobilized onto chitosan and glutaraldehyde-pretreated chitosan powders. The prepared biocatalysts were assayed in the direct esterification of oleic acid and ethanol to produce the ethyl oleate. In order to maximize ester production and avoid solvent-related costs (the solvent itself, solvent recovery and solvent recycle), the synthesis of ethyl oleate was performed in a solvent-free system.The different structures of the lipases chosen for the catalysis led to very different activity levels, with C. antarctica B derivatives being the most active ones. The parametric study performed revealed that the best operation conditions for ester synthesis are found at mild temperatures (35–45 °C), and in “biphasic systems” (two liquid phases), generated upon addition of relatively high quantities of water to the mixture of substrates. The reduction of the concentration of water in the organic reactive phase of biphasic systems favored ester synthesis, with higher ester yields in the first hours of reaction than those measured in systems with no added water.In the optimum conditions mentioned the biocatalyst resulting from the immobilization of lipase from C. antarctica B onto untreated chitosan powder led to 75% conversion of the fatty acid in 24 h of reaction. The stability of this catalyst also proved to be very attractive with five consecutive 24 h uses with a residual activity of 90–95%.

Keywords
Esterification; Lipase; Immobilization; Chitosan
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Chitosan-immobilized lipases for the catalysis of fatty acid esterifications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 769–777
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us