fulltext.study @t Gmail

Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy

Paper ID Volume ID Publish Year Pages File Format Full-Text
18039 42710 2007 8 PDF Available
Title
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy
Abstract

Optimization of lipase-catalyzed production of industrially important isoamyl acetate was carried out by response surface methodology (RSM). Isoamyl alcohol which was distillated from fusel oil and acetic acid were used as the substrates. Immobilized lipase from Candida antarctica (Novozym 435) was catalyzed to this reaction in the solvent-free system. RSM based on five-level, four variable central composite rotatable design was used to evaluate the effects of important parameters on the production of isoamyl acetate. The parameters are acid/alcohol mole ratio (0.2–0.8), enzyme amount (4–12%, w/w), temperature (30–50 °C) and reaction time (4–8 h). It was found that the most effective parameter was acid/alcohol mole ratio. The model indicated the optimum conditions for maximum esterification (4.4 mmol ester/g mixture) in the acid/alcohol mole ratio of 0.8 for 12% (w/w) enzyme at 30 °C and after 8 h, which were in good agreement with the experimental value. The coefficient of determination (R2) for the model is 0.9968. Probability value (P < 0.0001) demonstrates a very high significance for the regression model.

Keywords
Fusel oil; Isoamyl acetate; Optimization; Response surface methodology; Lipase; Solvent-free system
First Page Preview
Enzymatic esterification of isoamyl alcohol obtained from fusel oil: Optimization by response surface methodolgy
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 778–785
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us