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Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands

Paper ID Volume ID Publish Year Pages File Format Full-Text
18040 42710 2007 8 PDF Available
Title
Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands
Abstract

The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 °C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 μm) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150–160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples.

Keywords
Bacteriocin; Integrated product adsorption; Macroporous monolith; Cryogel in microtiter plate
First Page Preview
Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 786–793
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering