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Hydroxylation and epoxidation reactions catalyzed by CYP153 enzymes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18043 42710 2007 7 PDF Available
Title
Hydroxylation and epoxidation reactions catalyzed by CYP153 enzymes
Abstract

Several cytochrome P450 alkane hydroxylases of the CYP153 family were recently identified and functionally expressed in Pseudomonas putida GPo12 [van Beilen JB, Funhoff EG, van Loon A, Just A, Kaysser L, Bouza M, et al. Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases. Appl Environ Microbiol 2006;72:59–65]. Eight recombinants containing divergent CYP153's were cultivated on a 1.5–1.8 L scale with octane or decane/dodecane as sole carbon source. Growth rates between 0.06 and 0.23 h−1 were obtained and analysis of cell free extracts showed that the CYP153's were expressed to a maximum of 11% of total protein. The hydroxylation activities towards various substrates translate to maximal CYP153 turnover numbers between <0.1 and 58 min−1. Aliphatic alkanes were exclusively hydroxylated on terminal carbon atoms. Epoxidation experiments with styrene, octene and cyclohexene showed turnover numbers that were slightly lower than the hydroxylation activities, with the exception of CYP153A11 and CYP153A14. The results provide insight in structural features that determine the substrate specificity of these enzymes.

Keywords
P450; Epoxidation; Hydroxylation; CYP153; Biotransformation; Functional expression
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Hydroxylation and epoxidation reactions catalyzed by CYP153 enzymes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 806–812
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us