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Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase

Paper ID Volume ID Publish Year Pages File Format Full-Text
18055 42710 2007 6 PDF Available
Title
Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase
Abstract

In this paper we describe the production of acid phosphatase (ACP) by Fusarium venenatum A3/5 in phosphate-limited continuous flow culture at different phosphate concentrations, specific growth rates, and pH values. ACP production in F. venenatum was repressed by phosphate, and was only fully de-repressed at phosphate concentrations below 0.02 mM in the culture. The ACP was active from pH 2 to 7, and the activity could be attributed to one or possibly two isozymes, the one showing a strong activity maximum at pH 6, and the second showing a weaker activity maximum at pH 3. The specific ACP productivity was highest at dilution rates below D = 0.06 h−1. ACP production was also influenced by pH, which showed two, clearly separated production maxima at culture pH 3.3 and 5.8.

Keywords
Fusarium venenatum; Continuous flow culture; Acid phosphatase; Dilution rate; Phosphate concentration; pH
First Page Preview
Phosphate-limited continuous flow cultures of Fusarium venenatum A3/5 and production of acid phosphatase
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 4, 5 March 2007, Pages 902–907
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering