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A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants

Paper ID Volume ID Publish Year Pages File Format Full-Text
1806 91 2011 9 PDF Available
Title
A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants
Abstract

Current cardiovascular therapies are limited by the loss of endothelium, restenosis and thrombosis. The goal of this study was to develop a biomimetic hybrid nanomatrix that combined the unique properties of electrospun polycaprolactone (ePCL) nanofibers with self-assembled peptide amphiphiles (PAs). ePCL nanofibers have interconnected nanoporous structures, but are hampered by a lack of surface bioactivity to control cellular behavior. It has been hypothesized that PAs could self-assemble onto the surface of ePCL nanofibers and endow them with the characteristic properties of native endothelium. The PAs, which comprised hydrophobic alkyl tails attached to functional hydrophilic peptide sequences, contained enzyme-mediated degradable sites coupled to either endothelial cell-adhesive ligands (YIGSR) or polylysine (KKKKK) nitric oxide (NO) donors. Two different PAs (PA–YIGSR and PA–KKKKK) were successfully synthesized and mixed in a 90:10 (YK) ratio to obtain PA–YK. PA–YK was reacted with pure NO to develop PA–YK–NO, which was then self-assembled onto ePCL nanofibers to generate a hybrid nanomatrix, ePCL–PA–YK–NO. Uniform coating of self-assembled PA nanofibers on ePCL was confirmed by transmission electron microscopy. Successful NO release from ePCL–PA–YK–NO was observed. ePCL–YK and ePCL–PA–YK–NO showed significantly increased adhesion of human umbilical vein endothelial cells (HUVECs). ePCL–PA–YK–NO also showed significantly increased proliferation of HUVECs and reduced smooth muscle cell proliferation. ePCL–PA–YK–NO also displayed significantly reduced platelet adhesion compared with ePCL, ePCL–PA–YK and a collagen control. These results indicate that this hybrid nanomatrix has great potential application in cardiovascular implants.

Keywords
Cardiovascular; Electrospun; Nanomatrix; Nitric oxide; Endothelium
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A hybrid biomimetic nanomatrix composed of electrospun polycaprolactone and bioactive peptide amphiphiles for cardiovascular implants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 1, January 2011, Pages 225–233
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us