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Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil

Paper ID Volume ID Publish Year Pages File Format Full-Text
18080 42711 2007 6 PDF Available
Title
Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil
Abstract

Enzymatic synthesis of sn-1,3-diacylglycerols (sn-1,3-DAG) in two steps without isolation of the intermediates was investigated. Firstly ethanolysis of extra virgin olive oil (EVO) using immobilized non-regiospecific lipase from Candida antarctica (Novozym 435) was carried out to obtain glycerol (Gly) and fatty acid ethyl esters (FAEE). In the second step the ethanolysis products have been re-esterificated testing different sn-1,3-regiospecific lipases, both immobilized and non-immobilized, in different reaction media, that is in the presence of solvents or in a solvent-free system, for different times, at different temperatures (12, 25 and 40 °C). The lipase from Rhizomucor miehei (Lipozyme IM) has been the most effective among the sn-1,3-specific lipases screened.

Keywords
sn-1,3-Diacylglycerols; Extra virgin olive oil; Novozym 435; Lipozyme IM
First Page Preview
Biocatalysed synthesis of sn-1,3-diacylglycerol oil from extra virgin olive oil
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issues 6–7, 1 November 2007, Pages 727–732
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering