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Effects of carrier, sterilisation method, and incubation on survival of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants

Paper ID Volume ID Publish Year Pages File Format Full-Text
18087 42711 2007 5 PDF Available
Title
Effects of carrier, sterilisation method, and incubation on survival of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants
Abstract

The production and quality of rhizobial inoculants in many developing countries is limited by the availability of suitable carriers or technological limitations. Experiments were conducted to evaluate the potential of various inexpensive and widely available carrier materials. The carriers, evaluated, were: perlite with pH adjusted with calcium carbonate or charcoal, 1:4 mixtures of perlite and malt residue, sugarcane bagasse, coal, and rice husk. We also contrasted sterilisation procedures (autoclaving or gamma irradiation) and incubation after injection (with or without initial two weeks incubation at 28 °C) for these various carriers. Survival of Bradyrhizobium japonicum strain CB1809 was monitored over a period of 6 months upon storage at 4 °C. Most carriers evaluated, were able to maintain rhizobial populations of more than 1 × 109 rhizobia per gram of inoculant over that time period, with mixtures of perlite with either sugarcane bagasse or malt residue supporting the largest rhizobial populations and a mixture of perlite and rice husk the lowest. All carriers supported rhizobial growth over the 6 months period. Initially, rhizobial populations were greater with gamma irradiation than autoclaving, however after 6 months, this response was significant only with the perlite and sugarcane bagasse mixture. The incubation of the inoculant after injection also ultimately did not benefit rhizobial levels for any of the carriers, tested. Using simple sterilisation procedures and without incubating after injection, perlite based carriers can produce high quality inexpensive inoculants, maintaining bacterial populations of more than 1 × 109/g rhizobia for at least 6 months.

Keywords
Bradyrhizobium japonicum; Carrier; Incubation; Soybean (Glycine max L.) inoculant; Sterilisation
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Effects of carrier, sterilisation method, and incubation on survival of Bradyrhizobium japonicum in soybean (Glycine max L.) inoculants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 41, Issues 6–7, 1 November 2007, Pages 780–784
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us